Q1. The procedure followed by pharmaceutical analyst includes pouring water into a cup and then placing dots on the pencil line. The analyst will then set a filter paper by ensuring that ink does not go beyond the water level.
Q2. In normal phase modes, the stationary phase is polar while the mobile phase of chromatography is non-polar. In reversed phase modes, mobile phase is polar while as the stationary phase of chromatography is non-polar.
Moreover, reversed phase modes use alkyl hydrocarbons in the stationary phase. Normal phase modes use organic moieties or silica with amino and cyano functional groups in stationary phases of chromatography.
Q3. Thin layer technique has difficulties in reproducing clear paints. To overcome this problem, one must automate the whole technique. Moreover, one may add extra liquid on the chromatography to get over-large spots.
Q4 Derivatization procedures can be used if the reaction is dependable and proceeds to completion. Moreover, these procedures are also applicable if the reaction is general. A reaction is general when it allows a wide range of substrates, thus reducing complicating interference.
Q5. The choice of hydrogen gas is a suitable one. This is because hydrogen gas is useful in testing the purity of any substance and in separating various components of mixture. Therefore, chromatography process will be efficient if hydrogen gas is applied.
Q6. Principles of a non-vacuum atmospheric pressure include separating mixtures by partitioning stationery and mobile phases. This will result in retention on the mobile phase, thus changing the separation.
Q7. Mobile phase consists of many groups of separatory methods. In this case, the mixture is separated by the attraction of different components of the stationary phase. The stationary phase composes of porous solid, which is full of metal tubes or walls of a capillary tube.
Q8. Capillary electrophoresis is applicable when there is separation of ionic species. These species are separated by their frictional forces, charge, and hydrodynamic radius. Capillary electrophoresis is based on the principle that electrically charge analytes move under the control of an electric field. Furthermore, capillary electrophoresis separate species based on the principle of size to charge ratio…
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